核酸相關(guān)

菌種與質(zhì)粒>菌種與培養(yǎng)；病毒構(gòu)建；感受態(tài)及制備；原核載體；真核載體；報(bào)告基因質(zhì)粒；基因表達(dá)質(zhì)粒熒光探針質(zhì)粒；植物用質(zhì)粒；質(zhì)粒平臺(tái)

DNA提取與純化>質(zhì)粒抽提；DNA純化膠回收；基因組DNA抽提

DNA操作>內(nèi)切酶與外切酶；修飾酶；DNA連接；重組與克?。换蛲蛔?；基因編輯

反轉(zhuǎn)錄與PCR>反轉(zhuǎn)錄；PCR相關(guān)；定量PCR；等溫?cái)U(kuò)增

DNA電泳>電泳介質(zhì)與溶液；DNA分子量標(biāo)準(zhǔn)；電泳染料

DNA標(biāo)記和檢測(cè)>EMSA相關(guān)；建庫(kù)與高通量測(cè)序

RNA相關(guān)>RNA提??；切割、合成、連接和修飾；RNA保護(hù)；RNA病毒；反轉(zhuǎn)錄；建庫(kù)與高通量測(cè)序

多肽與蛋白

多肽>多肽激素；活性多肽；多肽底物；競(jìng)爭(zhēng)性多肽

重組蛋白>細(xì)胞因子；趨化因子；激素；抗體純化；重組蛋白酶相關(guān)；其他重組蛋白

酶及活性檢測(cè)>蛋白酶；酶活性檢測(cè)

蛋白樣品制備>裂解及蛋白抽提；蛋白酶抑*制劑；磷酸酶抑*制劑；蛋白修飾酶抑*制劑

蛋白表達(dá)與純化>原核蛋白表達(dá)；蛋白純化

蛋白檢測(cè)>蛋白定量；蛋白電泳；蛋白分子量標(biāo)準(zhǔn)；Western檢測(cè)；免疫沉淀；免疫染色；ELISA

細(xì)胞相關(guān)

細(xì)胞株>人細(xì)胞株；小鼠細(xì)胞株；其他細(xì)胞株

細(xì)胞組織培養(yǎng)>培養(yǎng)液相關(guān)；血清；細(xì)胞組織消化；原代細(xì)胞培養(yǎng)；支原體檢測(cè)與清除；細(xì)胞凍存；細(xì)胞培養(yǎng)耗材

基因表達(dá)調(diào)控>細(xì)胞轉(zhuǎn)染；病毒感染

細(xì)胞樣品制備>細(xì)胞組分分離；蛋白樣品制備；RNA樣品制備；DNA樣品制備

細(xì)胞增殖與分化>細(xì)胞增殖；細(xì)胞分化；細(xì)胞衰老

細(xì)胞死亡與自噬>細(xì)胞凋亡；細(xì)胞壞死；細(xì)胞焦亡；自噬

細(xì)胞組織染色>普通染色；熒光染色；免疫染色

信號(hào)傳導(dǎo)

信號(hào)小分子檢測(cè)>活性氧相關(guān)；一氧化氮相關(guān)；代謝小分子相關(guān)；鈣離子等檢測(cè)

轉(zhuǎn)錄調(diào)控>報(bào)告基因相關(guān)；EMSA相關(guān)

熒光探針>小分子熒光探針；細(xì)胞凋亡熒光探針；細(xì)胞器熒光探針；其他熒光探針

抗體

抗體制備>免疫佐劑；抗體純化

二抗>HRP標(biāo)記二抗；Biotin標(biāo)記二抗；ALP標(biāo)記二抗；熒光標(biāo)記二抗

常用一抗>內(nèi)參抗體；標(biāo)簽抗體；細(xì)胞器標(biāo)志抗體；細(xì)胞標(biāo)志抗體；對(duì)照IgG；敲除驗(yàn)證抗體；修飾抗體

凋亡與自噬>Regulation of Apoptosis Inhibition of Apoptosis；Death Receptor Signaling；Mitochondrial Control of Apoptosis；Autophagy Signaling；p53 Signaling

細(xì)胞代謝>Insulin Signaling；AMPK Signaling；Warburg Effect；Hypoxia Signaling；Glucose metabolism；Fatty acid metabolism；Endocrinology & Hormones；Transmembrane Transporters；Glutamine Metabolism；Other Metabolism

染色質(zhì)/表觀遺傳/細(xì)胞周期>Protein Acetylation；Histone Methylation；DNA Methylation；Crosstalk Between Protein Modifications；G1/S Checkpoint；G2M/DNA Damage Checkpoint；RNA Editing/Splicing；Epigenetics；DNA Damage and Repair；Nuclear Receptor Signaling；Chromatin Regulation

細(xì)胞骨架/細(xì)胞外基質(zhì)>Microtubule Dynamics；Actin Dynamics；Adherens Junction Dynamics；Cytoskeletal Signaling

免疫與炎癥>Jak/Stat:IL-6 Signaling；NF-κB Signaling；Toll-Like Receptor Signaling；B Cell Receptor Signaling；T-Cell Receptor Signaling；Cytokines；Chemokines；Tumor Necrosis Factor；Anti-infection；Interferon；T-Cell/B-Cell Activation；Macrophages；Complement Activation Pathway；Other Immune System

MAPK與PI3K/Akt通路>MAPK/Erk in Growth & Differentiation；MAPK/Erk in GPCR Signaling；SAPK/JNK Signaling；p38 MAPK Signaling；PI3K/Akt Signaling；mTOR Signaling；MAP Kinase；Protein Tyrosine Kinase Signaling

神經(jīng)科學(xué)>Amyloid and Neurofibrillary Tangle；Dopamine Signaling；Presynaptic Vesicle Trafficking；Neuronal Signaling

PKC、鈣離子及脂信號(hào)通路>PKC Signaling；Phospholipase Signaling

干細(xì)胞、發(fā)育與分化>Hippo Signaling Pathway；ESC Pluripotency and Differentiation；Stem Cell & Lineage Markers；Wnt/β-Catenin Signaling；Notch Signaling；Hedgehog Signaling；TGF-β Signaling；Angiogenesis

蛋白翻譯、折疊和降解>Translational Control；eIF4E and p70 S6K pathway；eIF2 pathway；Ubiquitin/Proteasome；Protein Folding

腫瘤研究>Breast Cancer

其它類(lèi)別一抗>Nuclear Receptor Signaling；ErbB/HER Signaling；Angiogenesis；Organelle Markers；Coagulation；Growth Factors；Transcription Factors；Signal Transduction；Others

抑制劑激活劑等

化合物庫(kù)>藥物庫(kù)

凋亡與自噬>Regulation of Apoptosis；Inhibition of Apoptosis；Death Receptor Signaling；Mitochondrial Control of Apoptosis；Autophagy Signaling

細(xì)胞代謝>Insulin Signaling；AMPK Signaling；Warburg Effect；Hypoxia Signaling；Glucose metabolism；Fatty acid metabolism；Transmembrane Transporters

染色質(zhì)/表觀遺傳/細(xì)胞周期>Protein Acetylation；Histone Methylation；Crosstalk Between Protein Modifications；G1/S Checkpoint；G2M/DNA Damage Checkpoint

細(xì)胞骨架/細(xì)胞外基質(zhì)>Microtubule Dynamics；Actin Dynamics；Adherens Junction Dynamics

免疫與炎癥>Jak/Stat:IL-6 Signaling；NF-κB Signaling；Toll-Like Receptor Signaling；B Cell Receptor Signaling；T-Cell Receptor Signaling；Antimicrobial Signaling

MAPK與PI3K/Akt通路>MAPK/Erk in Growth & Differentiation；MAPK/Erk in GPCR Signaling；SAPK/JNK Signaling；p38 MAPK Signaling；PI3K/Akt Signaling；mTOR Signaling；Protein Tyrosine Kinase

神經(jīng)科學(xué)>Dopamine Signaling；Presynaptic Vesicle Trafficking；Neurotransmission

PKC、鈣離子及脂信號(hào)通路>PKC Signaling；Phospholipase Signaling

干細(xì)胞、發(fā)育與分化>Wnt/β-Catenin Signaling；Notch Signaling；Hedgehog Signaling；TGF-β Signaling

蛋白翻譯、折疊和降解>Translational Control；Ubiquitin/Proteasome

其它類(lèi)別抑制劑激活劑>Nuclear Receptor Signaling；ErbB/HER Signaling；Angiogenesis；Coagulation；Anti COVID

常用試劑

生化試劑>糖類(lèi)；氨基酸與蛋白；蛋白；脂類(lèi)；核酸相關(guān)；輔酶；代謝物；激素；維生素；微生物培養(yǎng)；顯色劑；去垢劑；螯合劑；溶劑；氧化還原試劑；其它生化試劑；分離純化試劑；造模試劑；染色劑；校正溶液

常用酶>核酸酶；蛋白酶；其它常用酶

抗生素與防腐劑>哺乳動(dòng)物；細(xì)胞培養(yǎng)；細(xì)菌培養(yǎng)；真菌培養(yǎng)；防腐劑

緩沖液>電泳相關(guān)緩沖液；pH緩沖液；緩沖試劑；分子克隆相關(guān)緩沖液

儲(chǔ)存液>鈉鹽溶液；其它儲(chǔ)存液

植物與培養(yǎng)>植物培養(yǎng)

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